Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines.Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.Since applications vary, each investigator should titrate the reagent to obtain optimal results.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at.Please refer to to access safety data sheets (SDS).Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions.An isotype control should be used at the same concentration as the antibody of interest.Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.Please refer to for technical protocols. However, verification testing has not been performed on all conjugate lots. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity.Researchers should determine the optimal concentration of this reagent for their individual applications.This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. 555899), has been observed when the antibody conjugate was present during the lysis procedure. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat.
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